ABSTRACT
Like many other organizations in Canada and globally, nonprofit organizations have not been insulated from the negative effects of the COVID-19 pandemic. It has affected Canadian nonprofit organizations in numerous ways. This ranges from the effects of COVID-19 on the health of workers and clients to its effect on revenue. As predominantly essential service providers, nonprofit organizations have to find ways to continue operations during the COVID-19 pandemic to ensure that no one is left to fall through the cracks in an uncertain economy. © 2021, University of Alberta Library. All rights reserved.
ABSTRACT
Applications of small-angle scattering (SAS) in structural biology have benefited from continuing developments in instrumentation, tools for data analysis, modeling capabilities, standards for data and model presentation, and data archiving. The interplay of these capabilities has enabled SAS to contribute to advances in structural biology as the field pushes the boundaries in studies of biomolecular complexes and assemblies as large as whole cells, membrane proteins in lipid environments, and dynamic systems on time scales ranging from femtoseconds to hours. This review covers some of the important advances in biomolecular SAS capabilities for structural biology focused on over the last 5 years and presents highlights of recent applications that demonstrate how the technique is exploring new territories.
Subject(s)
Membrane Proteins/chemistry , X-Ray Diffraction/methods , Models, Molecular , Scattering, Small AngleABSTRACT
Résumé Depuis 2011, le juge des libertés et de la détention (JLD) est compétent pour contrôler les mesures de soins sans consentement. Ce contrôle bien qu’indispensable afin d’éviter les privations de liberté abusives est insuffisant. Face à la crise sanitaire, les lacunes de ce contrôle sont encore plus perceptibles dans un contexte d’atteinte aux droits des personnes hospitalisées sans consentement. Since 2011, the judge of freedoms and detention (JLD) is competent to control care measures without consent. This control, although essential in order to avoid abusive deprivation of liberty, is incomplete. Faced with the health crisis, the shortcomings of this control are more noticeable in a context of violation of the rights of people hospitalized without detriment.
ABSTRACT
This article analyzes the digital communication of Médecins Sans Frontières (MSF) during its intervention in Spain due to the emergency caused by COVID-19. Using content analysis techniques, the text engages with a number of the messages posted on the MSF website, emails sent to partners and publications on Facebook and Twitter. The material (a total 58 messages) is categorized by subject, sender, format, and objective, as well as the presence of informative or emotional approaches. Complementarily, items such as intensity, positive or negative undertones and the terms most frequently used are also examined. Our conclusion points towards the use of digital communication with a view to reinforce MSF's public image as an entity specialized in health care in emergency situations. The unprecedented intervention in Spain during the COVID-19 crisis has also shown MSF to be accountable to its audiences, showing how the organization usually works in other countries. © 2021, Rey Juan Carlos University. All rights reserved.
ABSTRACT
In the context of the Covid-19 pandemic, teleconsultation is an obvious solution in psychiatry to ensure continuity of care and facilitate access. However, the digitisation of ambulatory practices raises a certain number of reservations, in particular concerning the remote management of psychiatric emergencies. These situations, because of the specific aspects they cover, are in fact upsetting texts and recommendations of good practice in terms of teleconsultation. Thus, the questions of eligibility of people suffering from psychiatric disorders, the identification of an immediate self- or hetero-aggressive risk during teleconsultation and the establishment of a measure of psychiatric care without consent at the end of a teleconsultation require specific reflection in order to allow the practitioner to anticipate and manage the situation in the best possible way.
Subject(s)
COVID-19 , Remote Consultation , Emergencies , Humans , Pandemics , SARS-CoV-2ABSTRACT
The replication transcription complex (RTC) from the virus SARS-CoV-2 is responsible for recognizing and processing RNA for two principal purposes. The RTC copies viral RNA for propagation into new virus and for ribosomal transcription of viral proteins. To accomplish these activities, the RTC mechanism must also conform to a large number of imperatives, including RNA over DNA base recognition, basepairing, distinguishing viral and host RNA, production of mRNA that conforms to host ribosome conventions, interfacing with error checking machinery, and evading host immune responses. In addition, the RTC will discontinuously transcribe specific sections of viral RNA to amplify certain proteins over others. Central to SARS-CoV-2 viability, the RTC is therefore dynamic and sophisticated. We have conducted a systematic structural investigation of three components that make up the RTC: Nsp7, Nsp8, and Nsp12 (also known as RNA-dependent RNA polymerase). We have solved high-resolution crystal structures of the Nsp7/8 complex, providing insight into the interaction between the proteins. We have used small-angle x-ray and neutron solution scattering (SAXS and SANS) on each component individually as pairs and higher-order complexes and with and without RNA. Using size exclusion chromatography and multiangle light scattering-coupled SAXS, we defined which combination of components forms transient or stable complexes. We used contrast-matching to mask specific complex-forming components to test whether components change conformation upon complexation. Altogether, we find that individual Nsp7, Nsp8, and Nsp12 structures vary based on whether other proteins in their complex are present. Combining our crystal structure, atomic coordinates reported elsewhere, SAXS, SANS, and other biophysical techniques, we provide greater insight into the RTC assembly, mechanism, and potential avenues for disruption of the complex and its functions.